Intracytoplasmic sperm injection-produced equine embryos: transport, thawing, and transfer
Abstract
Intracytoplasmic sperm injection (ICSI)-produced equine embryos are transferred at the early blastocyst stage, similar to a day 6.5 in vivo-produced embryo and will benefit from a recipient mare that ovulated 3 - 5 days before transfer. Frozen-thawed and vitrified-thawed embryos may have an even narrower window of synchrony with recipients for best results. Day of blastocyst formation is a useful indicator of embryo quality and potential for establishing a successful pregnancy; early embryonic loss is higher for blastocysts that formed on days 9 and 10 after ICSI than those formed earlier.
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