Recovery, storage, and preparation of epididymal and vas deferens sperm for insemination, cryopreservation, and shipping
Abstract
Current technology allows preservation of sperm from testes and the distal reproductive tract if harvested after death determined largely by environmental and handling conditions (e.g. how long sperm remain viable within their nurturing structures postmortem) after death. Pregnancies are established using nonejaculated and even immotile sperm with appropriate preparation. There are very simple techniques available that can be employed by any practitioner to recover and prepare sperm from testes and the distal reproductive tract. This paper focuses on techniques for recovery, storage, and preparation of epididymal and vas deferens sperm, and techniques for shipping and cryopreservation.
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References
2. Stilley K: Survival of canine epididymal sperm under cooled and frozen-thawed conditions. Louisiana State University Master’s thesis 3830. Agricultural and Mechanical College; 2001.
3. Hassan H, Domain G, Luvoni G, et al: Canine and feline epididymal semen – a plentiful source of gametes. Animals (Basel) 2021;11:2961. https://doi.org/10.3390/ani11102961
4. Barker CA: Low temperature preservation of bovine epididymal spermatozoa. Can J Comp Med Vet Sci 1954;18:390–393.
5. Barker CA, Gandier JC: Pregnancy in a mare resulting from frozen epididymal spermatozoa. Can J Comp Med Vet Sci 1957;21:47–51.
6. Nagai T, Takahashi T, Masuda H, et al: In-vitro fertilization of pig oocytes by frozen boar spermatozoa. J Reprod Fertil 1988;84: 585–591. https://doi.org/10.1530/jrf.0.0840585
7. Martinez-Fresneda L, Castano C, Boveda P, et al: Epididymal and ejaculated sperm differ on their response to the cryopreservation and capacitation processes in mouflon (Ovis musimon). Sci Rep 2019;9:15659. https://doi.org/10.1038/s41598-019-52057-0
8. Nagy ZP, Liu J, Joris H, et al: The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod 1995;10:1123–1129. https://doi.org/10.1093/oxfordjournals.humrep.a136104
9. Bourne H, Watkins W, Speirs A, et al: Pregnancies after intracytoplasmic injection of sperm collected by fine needle biopsy of the testis. Fertil Steril 1995;64:433–436. https://doi.org/10.1016/S0015-0282(16)57747-4
10. Lin Y, Huang L, Seow K, et al: Intentional cryopreservation of epididymal spermatozoa from percutaneous aspiration for dissociated intracytoplasmic sperm injection cycles. Acta Obstet Gynecol Scand 2004;83:745–750. https://doi.org/10.1111/j.0001-6349.2004.00374.x
11. Franca L, Avelar G, Almeida F: Spermatogenesis and sperm transit through the epididymis in mammals with emphasis on pigs. Theriogenology 2005;63: 300–318. https://doi.org/10.1016/j.theriogenology.2004.09.014
12. Marchiani S, Tamburrino L, Muratopri M, et al: Epididymal sperm transport and fertilization. In: Simoni M, Huhtaniemi IT, editors. Endocrinology of the testis and male reproduction. New York City; Springer Publishing; 2017, p. 457–478.
13. Mataveia GA, Terblanche SJ, Nöthling JO, et al: Effect of heterologous seminal plasma and semen extenders on motility of frozen-thawed ram spermatozoa. J S Afr Vet Assoc. 2010;81:139–142. https://doi.org/10.4102/jsava.v81i3.135
14. Hori T, Hagiuda K, Kawakami E, et al: Unilateral intrauterine insemination with prostatic fluid-sensitized frozen caudal epididymal sperm in Beagle dogs. Theriogenology 2005;63:1573–1583. https://doi.org/10.1016/j.theriogenology.2004.05.027
15. Galarza D, Landi G, Mejia E, et al: Cryopreservation of dog epididymal spermatozoa by conventional freezing or ultra-rapid freezing with nonpermeable cryoprotectant. Cryobiology 2021;103:15–21. https://doi.org/10.1016/j.cryobiol.2021.10.002
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