Effect of egg yolk-free extender on canine sperm quality after cooling

Florencia  Gallelli; Cecilia  Allera; Evangelina  Moncalvo; Mercedes  Muir; Marcelo  Miragaya; Norma  Monachesi

doi:10.58292/CT.v18.13681

Florencia Gallelli Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina; and Catedra de Teriogenologia, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina
Cecilia Allera Catedra de Teriogenologia, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina
Evangelina Moncalvo Catedra de Teriogenologia, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina
Mercedes Muir Catedra de Teriogenologia, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina
Marcelo Miragaya Catedra de Teriogenologia, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina
Norma Monachesi Catedra de Teriogenologia, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina

DOI: https://doi.org/10.58292/CT.v18.13681

Keywords: Cryopreservation, extender, semen, dog

Abstract

Effectiveness of a commercial extender was compared to a conventional extender in cooling dog semen. Ejaculates (n = 21) from 7 dogs were evaluated (volume and pH, sperm motility, vigor, concentration, morphology, chromatin condensation, and membrane function). Samples were divided into 2 aliquots, centrifuged and the resulting pellet was diluted in the commercial extender without egg yolk or in the conventional extender (containing tris, citric acid, fructose, and egg yolk). Sperm progressive motility (PM), vigor, morphology, chromatin condensation, viability, and acrosomal integrity were assessed at 24, 48, and 96 hours after refrigeration at 4ºC. There were no differences in the percentage of morphologically normal sperm and sperm with condensed chromatin. However, percentage of live sperm with intact acrosomes was greater (p < 0.01) in samples diluted in the commercial extender. Furthermore, period of storage had a declining effect (p < 0.0001) only on PM for samples diluted in both extenders; PM was greater (p < 0.01) before cooling than at 48 and 96 hours of cooled storage and was greater (p < 0.05) at 24 hours storage than at 96 hours storage. In conclusion, the commercial extender was effective and better preserved acrosome integrity than the conventional extender.

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