Processing techniques for canine semen

Abstract

The use of artificial insemination with either cooled or frozen canine semen continues to gain in popularity world-wide. Processing and extension of canine semen is typically straightforward; however, sperm selection methods more commonly used in other species can be adapted for use with canine sperm. Processing after collection can be used to remove seminal plasma and prostatic fluid, to increase the concentration of the ejaculate, and to preferentially select normal sperm. Supplies and materials that may be helpful include a variable speed centrifuge, preferably with hanging buckets, plastic or glass 15mL conical tubes, disposable pipettes, flat-bevel needles, and larger-volume (1 mL to 5 mL) micropipettors and disposable tips.