Andrology laboratory review: evaluation of sperm motility

Abstract

This review is an effort by the andrology taskforce of the Association of Applied Animal
Andrology, American College of Theriogenologists, European College of Animal Reproduction, and
Society for Theriogenology. It is intended to serve as a reference on methods to evaluate sperm
motility in domestic animals and to contribute to adoption of best practices in veterinary andrology
laboratories and semen processing centers. Sperm motility evaluation topics covered include sample
preparation, subjective evaluation, computer-assisted semen analysis, and use of sperm quality
analyzers. Emphasis is given to principles of the methods, equipment, performing evaluation, and
common mistakes and/or pitfalls. In addition, precision and accuracy of the various methods are
discussed.