Embryo transfer and biosecurity: safeguarding healthy reproductive technologies in veterinary medicine

  • Carolina Rodrigues Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK, USA
  • Giovana Catandi Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK, USA
  • Reed Holyoak Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK, USA
DOI: https://doi.org/10.58292/CT.v18.14601
Keywords: Embryo handling procedures, international embryo trade, biosafety measures

Abstract

Embryo transfer (ET) is an essential reproductive biotechnology tool that supports genetic improvement, conservation programs and international germplasm trade across livestock species. Compared to the movement of live animals or semen, transport of properly collected and processed embryos presents a substantially lower biosecurity risk, owing to the protective role of intact zona pellucida, combined with standardized sanitary procedures established by the International Embryo Transfer Society (IETS) and the World Organization for Animal Health (WOAH). Rapid global expansion of in vitro produced (IVP) embryos, however, has introduced additional biosecurity considerations, as these embryos undergo multiple laboratory manipulations and are exposed to culture systems not encountered during in vivo development. Experimental evidence demonstrated that selected bacterial and intracellular pathogens may persist in IVP embryos following standard washing procedures under specific conditions; however, no confirmed outbreaks associated with commercially produced IVP embryos processed according to the standards have been reported. We summarize current evidence regarding pathogen transmission risks associated with ET; compare biosecurity characteristics of in vivo-derived (IVD) and IVP embryos; review current IETS and WOAH biosecurity guidelines; and discuss emerging challenges associated with continuing expansion of IVP technologies. Emphasis is placed on laboratory quality-control systems, donor health management and future research priorities needed to further strengthen international embryo biosecurity.

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References


1.
Pinto CR: Overview of Embryo Transfer in Farm Animals. Merck Veterinary Manual 2024. Available from: https://www.merckvetmanual.com/management-and-nutrition/embryo-transfer-in-farm-animals/overview-of-embryo-transfer-in-farm-animals [cited 06 July 2026].


2.
Thibier M, Guérin B: Embryo transfer in small ruminants: the method of choice for health control in germplasm exchanges. Livest Prod Sci 2000;62:253-270. doi: 10.1016/s0301-6226(99)00161-x


3.
Guérin B, Nibart M, Guienne BL, et al: Sanitary risks related to embryo transfer in domestic species. Theriogenology 1997;47:33-48. doi: 10.1016/s0093-691x(96)00337-8


4.
Givens MD, Marley SD: Approaches to biosecurity in bovine embryo transfer programs. Theriogenology 2008;69:129-136. doi: 10.1016/j.theriogenology.2007.09.009


5.
Hasler JF: Forty years of embryo transfer in cattle: a review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces. Theriogenology 2014;81:152-169. doi: 10.1016/j.theriogenology.2013.09.010


6.
Knutses IS, Serrano Y, Arksey F, et al: Comparison of in-vivo (fresh) and cryopreserved in-vitro produced embryo transfer programmes in clinical practice. Equine Vet J 2025;57:22-22. doi: 10.1111/evj.70014


7.
Souza-Fabjan JMG, Oliveira MEF, Guimarães MPP, et al: Review: non-surgical artificial insemination and embryo recovery as safe tools for genetic preservation in small ruminants. Animal 2023;17:100787. doi: 10.1016/j.animal.2023.100787


8.
Looney CR, Garcia-Guerra A, Larsen S: IETS Statistical Information Committee Report 2024 Data. Available from: https://www.iets.org [cited 06 July 2026].


9.
Leelawardana S, Perry G, Fiéni F, editors. Manual of the International Embryo Technology Society. 5th edition, Champaign, IL; International Embryo Technology Society: 2025. Available from: https://www.iets.org [cited 06 July 2026].


10.
World Organization for Animal Health (WOAH): Terrestrial Animal Health Code. Available from: https://www.woah.org/en/what-we-do/standards/codes-and-manuals/ [cited 06 July 2026].


11.
Bielanski A, Lalonde J: Efficacy of the International Embryo Transfer Society washing procedure for removing bovine viral diarrhea virus from oocytes matured in vitro. Theriogenology 2011;76:261-266. doi: 10.1016/j.theriogenology.2011.02.002


12.
Lonergan P, Fair T: In vitro-produced bovine embryos: dealing with the warts. Theriogenology 2008;69:17-22. doi: 10.1016/j.theriogenology.2007.09.007


13.
Panarace M, Aguero JI, Garnil C, et al: How healthy are clones and their progeny? Theriogenology 2007;67:70-75. doi: 10.1016/j.theriogenology.2006.09.036


14.
United States Department of Agriculture–APHIS: Bovine Germplasm Movement Plan (BGMP). Available from: https://www.securebeef.org/Assets/Bovine-Germplasm-Movement-Plan-FMD.pdf [cited 06 July 2026].


15.
Bielanski A: A review of the risk of contamination of semen and embryos during cryopreservation and measures to limit cross-contamination during banking to prevent disease transmission in ET practices. Theriogenology 2012;77:467-482. doi: 10.1016/j.theriogenology.2011.07.043


16.
Hyttel P, Sinowatz F, Vejlsted M, et al: Essentials of Domestic Animal Embryology. Edinburgh; Saunders Elsevier: 2010.


17.
Samuelsson B, Hultén F: Veterinary Embryology. Ames, IA; Wiley-Blackwell: 2014.


18.
Guerin B, Thibier M, Stringfellow DA: Sanitary risks related to embryo transfer. Theriogenology 1997;47:33-42. doi: 10.1016/S0093-691X(96)00337-9


19.
Wrathall AE: Embryo transfer and disease transmission in livestock. Reprod Domest Anim 1995;30:195-201. doi: 10.1016/0093-691x(94)00009-j


20.
Wrathall AE, Simmons HA, Van Soom A: Evaluation of risks of viral transmission to recipients of bovine embryos arising from fertilization with virus-infected semen. Theriogenology 2006;65:247-274. doi: 10.1016/j.theriogenology.2005.05.043


21.
Wrathall AE: Embryo transfer and disease transmission in livestock: a review of recent research. Theriogenology 1995;43:81-88. doi: 10.1016/0093-691x(94)00009-j


22.
Oriol JG, Betteridge KJ, Clarke AJ, et al: Mucin-like glycoproteins in the equine embryonic capsule. Mol Reprod Dev 1993;34:255-265. doi: 10.1002/mrd.1080340305


23.
Oriol JG: The equine embryonic capsule: practical implications of recent research. Equine Vet J 1994;26:184-186. doi: 10.1111/j.2042-3306.1994.tb04366.x


24.
Oriol JG, Sharom FJ, Betteridge KJ: Developmentally regulated changes in the glycoproteins of the equine embryonic capsule. Reproduction 1993;99:653-664. doi: 10.1530/jrf.0.0990653


25.
Tremoleda JL, Stout TAE, Lagutina I, et al: Effects of in vitro production on horse embryo morphology, cytoskeletal characteristics, and blastocyst capsule formation. Biol Reprod 2003;69:1895-1906. doi: 10.1095/biolreprod.103.018515


26.
Betteridge KJ: The structure and function of the equine capsule in relation to embryo manipulation and transfer. Equine Vet J 1989;21:92-100. doi: 10.1111/j.2042-3306.1989.tb04690.x


27.
Scott TJ, Carnevale EM, Maclellan LJ, et al: Embryo development rates after transfer of oocytes matured in vivo, in vitro, or within oviducts of mares. Theriogenology 2001;55:705-715. doi: 10.1016/s0093-691x(01)00438-1


28.
Bielanski A, Algire J, Lalonde A, et al: Transmission of bovine viral diarrhea virus (BVDV) via in vitro-fertilized embryos to recipients, but not to their offspring. Theriogenology 2009;71:499-508. doi: 10.1016/j.theriogenology.2008.08.015


29.
Bielanski A: A review of disease transmission studies in relationship to production of embryos by in vitro fertilization and to related new reproductive technologies. Biotechnol Adv 1997;15(3–4):633-656. doi: 10.1016/s0734-9750(97)00041-4


30.
Leese HJ, Sturmey RG, Whitear S-L: The oviductal environment and early embryo development: a question of supply and demand. Biol Reprod 2009;81:57-57. doi: 10.1093/biolreprod/81.s1.57


31.
Senn LK, Peterson KD, Edwards JL, et al: Oviduct and endometrial epithelium improve in vitro produced bovine embryo developmental kinetics. Reproduction 2024;167:e240008. doi: 10.1530/rep-24-0008


32.
Umair M, Scheeren VFDC, Beitsma MM, et al: In vitro-produced equine blastocysts exhibit greater dispersal and intermingling of inner cell mass cells than in vivo embryos. Int J Mol Sci 2023;24:9619. doi: 10.3390/ijms24119619


33.
Hinrichs K: In vitro production of equine embryos: state of the art. Reprod Domest Anim 2010;45:3-8. doi: 10.1111/j.1439-0531.2010.01624.x


34.
Lewis N, Schnauffer K, Hinrichs K, et al: Morphokinetics of early equine embryo development in vitro using time-lapse imaging, and use in selecting blastocysts for transfer. Reprod Fertil Dev 2019;31:1851-1861. doi: 10.1071/rd19225


35.
Lewis N, Canesin H, Choi YH, et al: Equine in vitro produced blastocysts: relationship of embryo morphology, stage and speed of development to foaling rate. Reprod Fertil Dev 2023;35:338-351. doi: 10.1071/rd22224


36.
Martin-Pelaez S, De La Fuente A, Takahashi K, et al: Abnormal cleavage patterns in equine in vitro-produced embryos lead to higher early pregnancy loss. Equine Vet J 2025:evj.70004. doi: 10.1111/evj.70004


37.
Pohjanvirta T, Vähänikkilä N, Mutikainen M, et al: Transmission of Mycoplasma bovis infection in bovine in vitro embryo production. Theriogenology 2023;199:43-49. doi: 10.1016/j.theriogenology.2023.01.011


38.
Van Soom A, Wrathall AE, Herrler A, et al: Is the zona pellucida an efficient barrier to viral infection? Reprod Fertil Dev 2009;22:21-31. doi: 10.1071/rd09230


39.
Alsaleh A, Pellerin JL, Rodolakis A, et al: Risk of Coxiella burnetii transmission via embryo transfer using in vitro early bovine embryos. Theriogenology 2014;81:849-853. doi: 10.1016/j.theriogenology.2013.12.015


40.
Pellerin J-L, Oseikria M, Moreno D, et al: Risk of Chlamydia abortus transmission via embryo transfer using in vitro produced early bovine embryos. Theriogenology 2019;126:114-120. doi: 10.1016/j.theriogenology.2018.11.033


41.
Rodolakis A, Laroucau K: Chlamydiaceae and chlamydial infections in sheep or goats. Vet Microbiol 2015;181:107-118. doi: 10.1016/j.vetmic.2015.07.010


42.
Givens MD: A clinical, evidence-based approach to infectious causes of infertility in beef cattle. Theriogenology 2006;66:648-654. doi: 10.1016/j.theriogenology.2006.04.021


43.
Timoney PJ, McCollum WH, Roberts AW, et al: Demonstration of the carrier state in naturally acquired equine arteritis virus infection in the stallion. Res Vet Sci 1986;41:279-280. doi: 10.1016/s0034-5288(18)30616-7


44.
Dean CJ, Hobgood AM, Blodgett GP, et al: The addition of ticarcillin-clavulanic acid to INRA 96 extender for stallion semen cooling. Equine Vet J 2012;44(S43):95-99. doi: 10.1111/j.2042-3306.2012.00638.x


45.
Santos CS, Silva AR: Current and alternative trends in antibacterial agents used in mammalian semen technology. Anim Reprod 2020;17:e20190111. doi: 10.21451/1984-3143-ar2019-0111


46.
Bielanski A, Nadin-Davis M, Sapp J, et al: Viral contamination of embryos and gametes in the bovine embryo industry. Cryobiology 2000;40:110-116. doi: 10.1006/cryo.1999.2227


47.
Delerue M, Breuil M-F, Duquesne F, et al: Acute endometritis due to Taylorella equigenitalis transmission by insemination of cryopreserved stallion semen. J Equine Vet Sci 2019;78:10-13. doi: 10.1016/j.jevs.2019.03.217


48.
Bielanski A, Dubuc C: In vitro fertilization of ova from cows experimentally infected with a non-cytopathic strain of bovine viral diarrhea virus. Anim Reprod Sci 1995;38:215-221. doi: 10.1016/0378-4320(95)98107-f


49.
Holyoak GR, Wang S, Liu G, et al: The effects of ceftiofur sodium on bovine oocyte and preimplantation embryonic development assessed by in vitro embryo production techniques. J Vet Pharmacol Ther 1998;21:92-98. doi: 10.1046/j.1365-2885.1998.00116.x


50.
Hernández-Avilés C, Love CC, Serafini R, et al: Inclusion of supplemental antibiotics (amikacin – penicillin) in a commercial extender for stallion semen: effects on sperm quality, bacterial growth, and fertility following cooled storage. Theriogenology 2020;158:209-217. doi: 10.1016/j.theriogenology.2020.09.018


51.
Brito LFC, Loomis PR, Klohonatz KM, et al: Penicillin and amikacin mixture has bactericidal activity equivalent to gentamicin, tylosin, lincomycin and spectinomycin mixture in equine frozen semen. Reprod Domest Anim 2023;58:349-357. doi: 10.1111/rda.14294


52.
Stringfellow DA, Wright JC: A review of the epidemiologic aspects of embryo transfer from Brucella abortus-infected cows. Theriogenology 1989;31:997-1006. doi: 10.1016/0093-691x(89)90483-4


53.
Cervantes MP, Palomino JM, Anzar M, et al: Effectiveness of washing procedures for removing Brucella abortus from in vivo- and in vitro-derived wood bison embryos. Theriogenol Wild 2026;100158. doi: 10.1016/j.therwi.2026.100158


54.
De Vos A, De Munck N: Trophectoderm biopsy: present state of the art. Genes 2025;16:134. doi: 10.3390/genes16020134


55.
Fernandes SLE, De Carvalho FAG: Preimplantation genetic testing: a narrative review. Porto Biomed J 2024;9. doi: 10.1097/j.pbj.0000000000000262


56.
Clark G, Babariya D, Kilbee M, et al: Investigating DNA contamination affecting embryo biopsy specimens taken for PGT-A: incidence and impact on the accuracy of chromosomal analysis. Reprod Biomed Online 2026;53:105637. doi: 10.1016/j.rbmo.2026.105637


57.
Wilsher S, Rigali F, Kovacsy S, et al: Puncture of the equine embryonic capsule and its repair in vivo and in vitro. J Equine Vet Sci 2020;93:103194. doi: 10.1016/j.jevs.2020.103194


58.
Barandalla M, Colleoni S, Perota A, et al: Preimplantation genetic testing in horses: biopsy of Piezo-ICSI embryos for sex, coat color, and disease alleles. Theriogenology 2025;246:117525. doi: 10.1016/j.theriogenology.2025.117525


59.
Thibier M, Perry G: IETS management of the challenges associated with embryo pathogen interaction. Reprod Fertil Dev 2023;36:16-26. doi: 10.1071/rd23144


60.
Funnell B, Briand-Amirat L, Viana JHM, et al: Disease risk of in vitro produced embryos: a review of current commercial practices in the context of international trade with emphasis on bovine embryos. Theriogenology 2024;230:212-219. doi: 10.1016/j.theriogenology.2024.09.019


61.
Haegeman A, Vandaele L, De Leeuw I, et al: Failure to remove bluetongue serotype 8 virus (BTV-8) from in vitro produced and in vivo derived bovine embryos and subsequent transmission of BTV-8 to recipient cows after embryo transfer. Front Vet Sci 2019;6:432. doi: 10.3389/fvets.2019.00432


62.
Marquant-Le Guienne B, Rémond M, Cosquer R, et al: Exposure of in vitro-produced bovine embryos to foot-and-mouth disease virus. Theriogenology 1998;50:109-116. doi: 10.1016/s0093-691x(98)00118-6


63.
Wrathall AE, Holyoak GR, Parsonson IM, et al: Risks of transmitting ruminant spongiform encephalopathies (prion diseases) by semen and embryo transfer techniques. Theriogenology 2008;70:725-745. doi: 10.1016/j.theriogenology.2008.05.049


64.
Wang S, Foote WC, Sutton DL, et al: Preventing experimental vertical transmission of scrapie by embryo transfer. Theriogenology 2001;56:315-327. doi: 10.1016/s0093-691x(01)00566-0


65.
Prell M, McGrath S, Kirkland P, et al: An investigation into the transmission and control of pestivirus in sheep in Australia. Aust Vet J 2024;102:60-66. doi: 10.1111/avj.13298


66.
Hebia I, Fiéni F, Duchamp G, et al: Potential risk of equine herpes virus 1 (EHV-1) transmission by equine embryo transfer. Theriogenology 2007;67:1485-1491. doi: 10.1016/j.theriogenology.2007.03.006


67.
Broaddus CC, Balasuriya UBR, Timoney PJ, et al: Infection of embryos following insemination of donor mares with equine arteritis virus infective semen. Theriogenology 2011;76:47-60. doi: 10.1016/j.theriogenology.2011.01.017


68.
Sherod JA, Wang S, Barnard D, et al: In vitro exposure of equine oocytes to equine arteritis virus. Theriogenology 1998;49:260. doi: 10.1016/s0093-691x(98)90613-6


69.
Holyoak GR, Balasuriya UBR, Timoney PJ, et al: Equine viral arteritis: current status and prevention. Theriogenology 2008;70:403-414. doi: 10.1016/j.theriogenology.2008.04.020


70.
Gregg K, Polejaeva I: Risk of equine infectious anemia virus disease transmission through in vitro embryo production using somatic cell nuclear transfer. Theriogenology 2009;72:289-299. doi: 10.1016/j.theriogenology.2009.03.009


71.
Thibier M, Stringfellow DA: Health and Safety Advisory Committee (HASAC) of the International Embryo Transfer Society (IETS) has managed critical challenges for two decades. Theriogenology 2003;59:1067-1078. doi: 10.1016/s0093-691x(02)01298-0


72.
Bielanski A, Devenish J, Phipps-Todd B: Effect of Mycoplasma bovis and Mycoplasma bovigenitalium in semen on fertilization and association with in vitro produced morula and blastocyst stage embryos. Theriogenology 2000;53:1213-1223. doi: 10.1016/s0093-691x(00)00266-1


73.
Pribylova R, Slana I, Cech S, et al: Mycobacterium avium subsp. paratuberculosis detected in the reproductive tract of cows from an infected herd. Reprod Domest Anim 2013;48:790-794. doi: 10.1111/rda.12163


74.
Kauffold J, Wehrend A, Sigmarsson H: Chlamydia and Chlamydophilia in bovine reproduction. Clinical Theriogenology 2014;6:251-254.


75.
Aymée L, Mendes J, Lilenbaum W: Bovine genital leptospirosis: an update of this important reproductive disease. Animals 2024;14:322. doi: 10.3390/ani14020322


76.
Cagnoli CI, Chiapparrone ML, Cacciato CS, et al: Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos. Vet Microbiol 2024;288:109925. doi: 10.1016/j.vetmic.2023.109925


77.
Oseikria M, Pellerin JL, Rodolakis A, et al: Can Chlamydia abortus be transmitted by embryo transfer in goats? Theriogenology 2016;86:1482-1488. doi: 10.1016/j.theriogenology.2016.05.006


78.
Alsaleh A, Fieni F, Rodolakis A, et al: Can Coxiella burnetii be transmitted by embryo transfer in goats? Theriogenology 2013;80:571-575. doi: 10.1016/j.theriogenology.2013.05.023


79.
Pellerin JL, Alsaleh A, Mermillod P, et al: Attachment of Coxiella burnetii to the zona pellucida of in vitro produced goat embryos. Theriogenology 2018;106:259-264. doi: 10.1016/j.theriogenology.2017.10.033


80.
Timoney PJ: HORSE SPECIES SYMPOSIUM: contagious equine metritis: an insidious threat to the horse breeding industry in the United States. J Anim Sci 2011;89:1552-1560. doi: 10.2527/jas.2010-3368


81.
Yao C: Tritrichomonas foetus infections in female beef cattle with abortion in Wyoming, USA. JMM Case Rep 2015;2. doi: 10.1099/jmmcr.0.000028


82.
Bielanski A, Ghazi DF, Phipps-Toodd B: Observations on the fertilization and development of preimplantation bovine embryos in vitro in the presence of Tritrichomonas foetus. Theriogenology 2004;61:821-829. doi: 10.1016/s0093-691x(03)00229-2


83.
Dubey JP, Lindsay DS: A review of Neospora caninum and neosporosis. Vet Parasitol 1996;67:1-59. doi: 10.1016/s0304-4017(96)01035-7


84.
Baillargeon P, Fecteau G, Paré J, et al: Evaluation of the Embryo Transfer Procedure Proposed by the International Embryo Transfer Society (IETS) as a Method of Controlling Vertical Transmission of Neospora caninum in Cattle. Am Assoc Bov Pract Conf Proc 2001:218:1803–1806. doi: 10.2460/javma.2001.218.1803


85.
Dubey JP: Toxoplasmosis of Animals and Humans. Boca Raton, FL; CRC Press: 2010.


86.
Consalter A, Silva AF, Frazão-Teixeira E, et al: Toxoplasma gondii transmission by artificial insemination in sheep with experimentally contaminated frozen semen. Theriogenology 2017;90:169-174. doi: 10.1016/j.theriogenology.2016.12.004


87.
Rothschild CM: Equine piroplasmosis. J Equine Vet Sci 2013;33:497-508. doi: 10.1016/j.jevs.2013.03.189


88.
Rothschild CM, Knowles DP: Equine piroplasmosis. In: Sellon DC, Long MT: editors. Equine Infectious Diseases. St. Louis; Elsevier: 2007. p. 465–473.


89.
Tamzali Y: Equine piroplasmosis: an updated review. Equine Vet Educ 2013;25:590-598. doi: 10.1111/eve.12070


90.
Farin PW, Farin CE: Transfer of bovine embryos produced in vivo or in vitro: survival and fetal development. Biol Reprod 1995;52:676-682. doi: 10.1095/biolreprod52.3.676


91.
He Y, Mao Y, Chen Y, et al: Effects of embryo microbial contamination on ART and neonatal outcomes. Infect Drug Resist 2024;17:4137-4148. doi: 10.2147/idr.s478594


92.
Sanioglu Golen G, Akar K, Karaşahin T, et al: Microbial contamination of in vitro-derived cattle embryos and resistance genes. Acta Vet (Beogr) 2025;75:326-338. doi: 10.2478/acve-2025-0025


93.
Borges ED, Berteli TS, Reis TF, et al: Microbial contamination in assisted reproductive technology: source, prevalence, and cost. J Assist Reprod Genet 2020;37:53-61. doi: 10.1007/s10815-019-01640-5


94.
Caneschi A, Bardhi A, Barbarossa A, et al: The use of antibiotics and antimicrobial resistance in veterinary medicine, a complex phenomenon: a narrative review. Antibiotics 2023;12:487. doi: 10.3390/antibiotics12030487


95.
Shisong C, Wrathall AE: The importance of the zona pellucida for disease control in livestock by embryo transfer. Br Vet J 1989;145:129-140. doi: 10.1016/0007-1935(89)90095-x


96.
Bielanski A, Lutze-Wallace CL, Nadin-Davis S: Adherence of bovine viral diarrhea virus to bovine oocytes and embryos with a hardened zona pellucida cultured in vitro. Can J Vet Res 2003;67:48-51.


97.
Daniel Givens M, Gard JA, Stringfellow DA: Relative risks and approaches to biosecurity in the use of embryo technologies in livestock. Theriogenology 2007;68:298-307. doi: 10.1016/j.theriogenology.2007.04.004


98.
Stringfellow DA, Lauerman LH, Nasti KB, et al: Trypsin treatment of bovine embryos after in vitro exposure to infectious bovine rhinotracheitis virus or bovine herpesvirus-4. Theriogenology 1990;33:331. doi: 10.1016/0093-691x(90)90001-a


99.
Alias AB, Huang H-Y, Yao D-J: A review on microfluidics: an aid to assisted reproductive technology. Molecules 2021;26:4354. doi: 10.3390/molecules26144354


100.
Alegretti JR, Rocha AMD, Nogueira-de-Souza NC, et al: Controlled dynamic microfluidic culture of murine, bovine, and human embryos improves development: proof-of-concept studies. Cells 2024;13:2080. doi: 10.3390/cells13242080


101.
Su Y-S, Tsai W-H, Wu H-C, et al: Multiplex metagenomic sequencing for rapid viral pathogen identification and surveillance in clinical specimens. BMC Infect Dis 2025;25:1531. doi: 10.1186/s12879-025-11952-w


102.
Zmuidinaite R, Sharara FI, Iles RK. Current advancements in noninvasive profiling of the embryo culture media secretome. Int J Mol Sci 2021;22:2513. doi: 10.3390/ijms22052513


103.
Park JH, Lee JH, Choi KM, et al: Rapid sexing of preimplantation bovine embryo using consecutive and multiplex polymerase chain reaction (PCR) with biopsied single blastomere. Theriogenology 2001;55:1843-1853. doi: 10.1016/s0093-691x(01)00526-x


104.
Yáñez Ramil U, Jezierska S, Krupa M, et al: Fundamentals of microbiome-based therapies for reproductive tract inflammatory diseases in domestic animals. Anim Reprod 2025;22:e20250030. doi: 10.1590/1984-3143-ar2025-0030


105.
Galvão KN, Bicalho RC, Jeon SJ: Symposium review: the uterine microbiome associated with the development of uterine disease in dairy cows. J Dairy Sci 2019;102:11786-11797. doi: 10.3168/jds.2019-17106
Published
2026-10-29
How to Cite
Rodrigues , C., Catandi , G., & Holyoak , R. (2026). Embryo transfer and biosecurity: safeguarding healthy reproductive technologies in veterinary medicine. Clinical Theriogenology, 18. https://doi.org/10.58292/CT.v18.14601
Issue
Vol. 18 (2026): Special Issue in progress: Advances and Clinical Applications of Embryo Transfer and Assisted Reproductive Technologies in Domestic and Exotic Animals
Section
Review Reports
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