Effects of a semen extender treated with coenzyme Q10-ubiquinol on the quality of thawed ram sperm
Keywords:
Antioxidants, reactive oxygen species, sperm, small ruminants
Abstract
Objective was to determine whether addition of coenzyme Q10 - ubiquinol to glycerol extender improved cryopreserved ram sperm quality. Semen was collected (electroejaculation) from mature Suffolk rams (n = 6) 6 times during the breeding season. Pooled semen from each session was processed and cryopreserved in extenders with 150 µmol/liter of ubiquinol (treated) and without (control). Thawed cryopreserved semen was evaluated using computer-assisted sperm analysis for motility and kinetic parameters. Sperm viability was determined via plasma membrane integrity. After thawing, measurements were obtained at 10, 20, 60, and 120 minutes. In treated samples, there were increases in progressive motility (p < 0.0001), curvilinear velocity (p = 0.002), straight line velocity (p = 0.003) and time average velocity (p = 0.001), and sperm tended (p = 0.07) to have more viability. Furthermore, there were time-related decreases in all variables in treated control and samples. Ubiquinol improved cryopreserved ram sperm quality.
Downloads
Download data is not yet available.
References
1. Gibbons AE, Fernandez J, Bruno-Galarraga MM, et al: Technical recommendations for artificial insemination in sheep. Anim Reprod 2019;16:803-809. doi: 10.21451/1984-3143-AR2018-0129
2. Salamon S, Maxwell WM: Storage of ram semen. Anim Reprod Sci 2000;62:77-111. doi: 10.1016/S0378-4320(00)00155-X
3. Aitken RJ, Baker MA, De Iuliis GN, et al: New insights into sperm physiology and pathology. In: Habenicht UF., Aitken RJ. (editors). Fertility Control. Handbook of Experimental Pharmacology, vol 198. Berlin, Springer: 2010; 198:99–115. doi: 10.1007/978-3-642-02062-9_7
4. Amidi F, Pazhohan A, Shabani Nashtaei M, et al: The role of antioxidants in sperm freezing: a review. Cell Tissue Bank 2016;17:745-756. doi: 10.1007/s10561-016-9566-5
5. Aitken RJ, Lambourne S, Gibb Z: The John Hughes Memorial Lecture: aspects of sperm physiology – oxidative stress and the functionality of stallion spermatozoa. J Equine Vet Sci 2014;34:17-27. doi: 10.1016/j.jevs.2013.10.120
6. Gvozdjáková A, Kucharská J, Dubravicky J, et al: Coenzyme Q10, α-tocopherol, and oxidative stress could be important metabolic biomarkers of male infertility. Dis Markers 2015;2015:827941. doi: 10.1155/2015/827941
7. Bailey JL, Bilodeau JF, Cormier N: Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. J Androl 2000;21:1-7. doi: 10.1002/j.1939-4640.2000.tb03268.x
8. Ernster L, Dallner G: Biochemical, physiological and medical aspects of ubiquinone function. Bba-Mol Basis Dis 1995;1271:195-204. doi: 10.1016/0925-4439(95)00028-3
9. Turunen M, Olsson J, Dallner G: Metabolism and function of coenzyme Q. Biochim Biophys Acta 2004;1660:171-199. doi: 10.1016/j.bbamem.2003.11.012
10. Lançoni R, Celeghini ECC, Giuli V, et al: Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm. Anim Reprod 2021;18:e20200218. doi: 10.1590/1984-3143-ar2020-0218
11. Gualtieri R, Barbato V, Fiorentino I, et al: Treatment with zinc, d-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development. Theriogenology 2014;82:592-598. doi: 10.1016/j.theriogenology.2014.05.028
12. Nogueira BG, Sampaio BF, Souza MI, et al: Coenzyme Q10 and alpha-tocopherol prevent the lipid peroxidation of cooled equine semen. Reprod Domest Anim 2015;50:1003-1010. doi: 10.1111/rda.12627
13. Yousefian I, Emamverdi M, Karamzadeh-Dehaghani A, et al: Attenuation of cryopreservation-induced oxidative stress by antioxidant: Impact of coenzyme Q(10) on the quality of post-thawed buck spermatozoa. Cryobiology 2018;81:88-93. doi: 10.1016/j.cryobiol.2018.02.005
14. Raouf SM, Taha AT: Effects of COQ10 with vitamin E supplementation on semen quality and seminal plasma parameters of broiler breeder males. Iraqi J Vet Sci 2021;35:65-70. doi: 10.33899/ijvs.2020.126287.1289
15. Wang Y, Kang Y, Zhang L, et al: Coenzyme Q(10) improves the quality of sheep sperm stored at room temperature by mitigating oxidative stress. Anim Sci J 2022;93:e13708. doi: 10.1111/asj.13708
16. Pastor-Maldonado CJ, Suarez-Rivero JM, Povea-Cabello S, et al: Coenzyme Q(10): novel formulations and medical trends. Int J Mol Sci 2020;21;8432. doi: 10.3390/ijms21228432
17. Uchida Y, Wakimoto K, Takahashi H, et al: The bioavailability of reduced coenzyme Q10 water-dispersive powder after single oral administration. J Altern Complement Med 2014;11:103-105. doi: 10.1625/jcam.11.103
18. Salah N, Sauvant D, Archimède H: Nutritional requirements of sheep, goats and cattle in warm climates: a meta-analysis. Animal 2014;8:1439-1447. doi: 10.1017/S1751731114001153
19. Carneiro JAM, Canisso IF, Bandeira RS, et al: Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability. Anim Reprod Sci 2018;192:107-118. doi: 10.1016/j.anireprosci.2018.02.020
20. Khalifa TA, Lymberopoulos AG, El-Saidy BE: Testing usability of butylated hydroxytoluene in conservation of goat semen. Reprod Domest Anim 2008;43:525-530. doi: 10.1111/j.1439-0531.2007.00947.x
21. Kumaresan A, Das Gupta M, Datta TK, et al: Sperm DNA integrity and male fertility in farm animals: a review. Front Vet Sci 2020;7:321. doi: 10.3389/fvets.2020.00321
22. Balercia G, Mosca F, Mantero F, et al: Coenzyme Q(10) supplementation in infertile men with idiopathic asthenozoospermia: an open, uncontrolled pilot study. Fertil Steril 2004;81:93-98. doi: 10.1016/j.fertnstert.2003.05.009
23. Masoudi R, Sharafi M, Shahneh AZ: Effects of CoQ10 on the quality of ram sperm during cryopreservation in plant and animal based extenders. Anim Reprod Sci 2019;208:106103. doi: 10.1016/j.anireprosci.2019.06.015
24. Hernandez-Camacho JD, Bernier M, Lopez-Lluch G, et al: Coenzyme Q(10) supplementation in aging and disease. Front Physiol 2018;9:44. doi: 10.3389/fphys.2018.00044
25. Saeed AM, El-Nagar HA, Wafa WM, et al: Effect of coenzyme Q10 as an antioxidant added to semen extender during cryopreservation of buffalo and cattle semen. J Anim Poult Prod 2016;7:403-408. doi: 10.21608/jappmu.2016.48748
26. Yousefian I, Zare-Shahneh A, Zhandi M: The effect of coenzyme Q10 and α-tocopherol in skim milk–based extender for preservation of Caspian stallion semen in cool condition. J Equine Vet Sci 2014;34:949-954. doi: 10.1016/j.jevs.2014.04.002
27. Pindaru L: Effects of coenzyme Q10 on sperm viability durign storage of boar semen at 17°C. Sci Works 2016;61:32-36.
28. Daghigh Kia H, Bolooki Z, Vaseghi Dodran H, et al: Effect of adding coenzyme Q10 and Ellagic acid during cryopreservation on post-thaw quality of ram semen. Iran J Appl Anim Sci 2017;7:445-451.
29. Gillan L, Evans G, Maxwell WM: Capacitation status and fertility of fresh and frozen-thawed ram spermatozoa. Reprod Fertil Dev 1997;9:481-487. doi: 10.1071/R96046
30. Castellini C, Dal Bosco A, Ruggeri S, et al: What is the best frame rate for evaluation of sperm motility in different species by computer-assisted sperm analysis? Fertil Steril 2011;96:24-27. doi: 10.1016/j.fertnstert.2011.04.096
31. Balasuriya A, Serhal P, Doshi A, et al: Processes involved in assisted reproduction technologies significantly increase sperm DNA fragmentation and phosphatidylserine translocation. Andrologia 2014;46:86-97. doi: 10.1111/and.12052
32. Srivastava N, Pande M: Mitochondrion: features, functions and comparative analysis of specific probes in detecting sperm cell damages. Asian Pac J Reprod 2016;5:445-452. doi: 10.1016/j.apjr.2016.10.008
33. Aitken RJ: Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol Reprod Dev 2017;84:1039-1052. doi: 10.1002/mrd.22871
34. Masoudi R, Sharafi M, Pourazadi L: Improvement of rooster semen quality using coenzyme Q10 during cooling storage in the Lake extender. Cryobiology 2019;88:87-91. doi: 10.1016/j.cryobiol.2019.03.003
2. Salamon S, Maxwell WM: Storage of ram semen. Anim Reprod Sci 2000;62:77-111. doi: 10.1016/S0378-4320(00)00155-X
3. Aitken RJ, Baker MA, De Iuliis GN, et al: New insights into sperm physiology and pathology. In: Habenicht UF., Aitken RJ. (editors). Fertility Control. Handbook of Experimental Pharmacology, vol 198. Berlin, Springer: 2010; 198:99–115. doi: 10.1007/978-3-642-02062-9_7
4. Amidi F, Pazhohan A, Shabani Nashtaei M, et al: The role of antioxidants in sperm freezing: a review. Cell Tissue Bank 2016;17:745-756. doi: 10.1007/s10561-016-9566-5
5. Aitken RJ, Lambourne S, Gibb Z: The John Hughes Memorial Lecture: aspects of sperm physiology – oxidative stress and the functionality of stallion spermatozoa. J Equine Vet Sci 2014;34:17-27. doi: 10.1016/j.jevs.2013.10.120
6. Gvozdjáková A, Kucharská J, Dubravicky J, et al: Coenzyme Q10, α-tocopherol, and oxidative stress could be important metabolic biomarkers of male infertility. Dis Markers 2015;2015:827941. doi: 10.1155/2015/827941
7. Bailey JL, Bilodeau JF, Cormier N: Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. J Androl 2000;21:1-7. doi: 10.1002/j.1939-4640.2000.tb03268.x
8. Ernster L, Dallner G: Biochemical, physiological and medical aspects of ubiquinone function. Bba-Mol Basis Dis 1995;1271:195-204. doi: 10.1016/0925-4439(95)00028-3
9. Turunen M, Olsson J, Dallner G: Metabolism and function of coenzyme Q. Biochim Biophys Acta 2004;1660:171-199. doi: 10.1016/j.bbamem.2003.11.012
10. Lançoni R, Celeghini ECC, Giuli V, et al: Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm. Anim Reprod 2021;18:e20200218. doi: 10.1590/1984-3143-ar2020-0218
11. Gualtieri R, Barbato V, Fiorentino I, et al: Treatment with zinc, d-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development. Theriogenology 2014;82:592-598. doi: 10.1016/j.theriogenology.2014.05.028
12. Nogueira BG, Sampaio BF, Souza MI, et al: Coenzyme Q10 and alpha-tocopherol prevent the lipid peroxidation of cooled equine semen. Reprod Domest Anim 2015;50:1003-1010. doi: 10.1111/rda.12627
13. Yousefian I, Emamverdi M, Karamzadeh-Dehaghani A, et al: Attenuation of cryopreservation-induced oxidative stress by antioxidant: Impact of coenzyme Q(10) on the quality of post-thawed buck spermatozoa. Cryobiology 2018;81:88-93. doi: 10.1016/j.cryobiol.2018.02.005
14. Raouf SM, Taha AT: Effects of COQ10 with vitamin E supplementation on semen quality and seminal plasma parameters of broiler breeder males. Iraqi J Vet Sci 2021;35:65-70. doi: 10.33899/ijvs.2020.126287.1289
15. Wang Y, Kang Y, Zhang L, et al: Coenzyme Q(10) improves the quality of sheep sperm stored at room temperature by mitigating oxidative stress. Anim Sci J 2022;93:e13708. doi: 10.1111/asj.13708
16. Pastor-Maldonado CJ, Suarez-Rivero JM, Povea-Cabello S, et al: Coenzyme Q(10): novel formulations and medical trends. Int J Mol Sci 2020;21;8432. doi: 10.3390/ijms21228432
17. Uchida Y, Wakimoto K, Takahashi H, et al: The bioavailability of reduced coenzyme Q10 water-dispersive powder after single oral administration. J Altern Complement Med 2014;11:103-105. doi: 10.1625/jcam.11.103
18. Salah N, Sauvant D, Archimède H: Nutritional requirements of sheep, goats and cattle in warm climates: a meta-analysis. Animal 2014;8:1439-1447. doi: 10.1017/S1751731114001153
19. Carneiro JAM, Canisso IF, Bandeira RS, et al: Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability. Anim Reprod Sci 2018;192:107-118. doi: 10.1016/j.anireprosci.2018.02.020
20. Khalifa TA, Lymberopoulos AG, El-Saidy BE: Testing usability of butylated hydroxytoluene in conservation of goat semen. Reprod Domest Anim 2008;43:525-530. doi: 10.1111/j.1439-0531.2007.00947.x
21. Kumaresan A, Das Gupta M, Datta TK, et al: Sperm DNA integrity and male fertility in farm animals: a review. Front Vet Sci 2020;7:321. doi: 10.3389/fvets.2020.00321
22. Balercia G, Mosca F, Mantero F, et al: Coenzyme Q(10) supplementation in infertile men with idiopathic asthenozoospermia: an open, uncontrolled pilot study. Fertil Steril 2004;81:93-98. doi: 10.1016/j.fertnstert.2003.05.009
23. Masoudi R, Sharafi M, Shahneh AZ: Effects of CoQ10 on the quality of ram sperm during cryopreservation in plant and animal based extenders. Anim Reprod Sci 2019;208:106103. doi: 10.1016/j.anireprosci.2019.06.015
24. Hernandez-Camacho JD, Bernier M, Lopez-Lluch G, et al: Coenzyme Q(10) supplementation in aging and disease. Front Physiol 2018;9:44. doi: 10.3389/fphys.2018.00044
25. Saeed AM, El-Nagar HA, Wafa WM, et al: Effect of coenzyme Q10 as an antioxidant added to semen extender during cryopreservation of buffalo and cattle semen. J Anim Poult Prod 2016;7:403-408. doi: 10.21608/jappmu.2016.48748
26. Yousefian I, Zare-Shahneh A, Zhandi M: The effect of coenzyme Q10 and α-tocopherol in skim milk–based extender for preservation of Caspian stallion semen in cool condition. J Equine Vet Sci 2014;34:949-954. doi: 10.1016/j.jevs.2014.04.002
27. Pindaru L: Effects of coenzyme Q10 on sperm viability durign storage of boar semen at 17°C. Sci Works 2016;61:32-36.
28. Daghigh Kia H, Bolooki Z, Vaseghi Dodran H, et al: Effect of adding coenzyme Q10 and Ellagic acid during cryopreservation on post-thaw quality of ram semen. Iran J Appl Anim Sci 2017;7:445-451.
29. Gillan L, Evans G, Maxwell WM: Capacitation status and fertility of fresh and frozen-thawed ram spermatozoa. Reprod Fertil Dev 1997;9:481-487. doi: 10.1071/R96046
30. Castellini C, Dal Bosco A, Ruggeri S, et al: What is the best frame rate for evaluation of sperm motility in different species by computer-assisted sperm analysis? Fertil Steril 2011;96:24-27. doi: 10.1016/j.fertnstert.2011.04.096
31. Balasuriya A, Serhal P, Doshi A, et al: Processes involved in assisted reproduction technologies significantly increase sperm DNA fragmentation and phosphatidylserine translocation. Andrologia 2014;46:86-97. doi: 10.1111/and.12052
32. Srivastava N, Pande M: Mitochondrion: features, functions and comparative analysis of specific probes in detecting sperm cell damages. Asian Pac J Reprod 2016;5:445-452. doi: 10.1016/j.apjr.2016.10.008
33. Aitken RJ: Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol Reprod Dev 2017;84:1039-1052. doi: 10.1002/mrd.22871
34. Masoudi R, Sharafi M, Pourazadi L: Improvement of rooster semen quality using coenzyme Q10 during cooling storage in the Lake extender. Cryobiology 2019;88:87-91. doi: 10.1016/j.cryobiol.2019.03.003
Published
2024-12-06
How to Cite
Arroyo E., Bayly W., Chakraborty S., Leadon D., & Tibary A. (2024). Effects of a semen extender treated with coenzyme Q10-ubiquinol on the quality of thawed ram sperm. Clinical Theriogenology, 16. https://doi.org/10.58292/CT.v16.10820
Issue
Section
Research Reports
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors retain copyright of their work, with first publication rights granted to Clinical Theriogenology. Read more about copyright and licensing here.
