Ovum pick up, in vitro embryo production, and embryo transfer as value-added theriogenology service in miniature Hereford cattle operation
Keywords:
Miniature Hereford, synchronization, ovum pick-up, in vitro, embryo transfer
Abstract
Goal was to provide evidence for adopting assisted reproductive technologies (ART), ovum pick up (OPU), in vitro embryo production (IVP), and embryo transfer (ET) in small beef cattle operations as value-added service. Day 7 embryos (n = 14) were produced via IVP utilizing oocytes (n = 87) collected by OPU from miniature Hereford donor cows (n = 3; mean age: 7 years) and fertilized with semen from miniature Hereford bulls. Irish Black (n = 8; 14 months) and Hereford (n = 1; 20 months) recipient heifers were synchronized using 5-day Select-Synch + controlled internal drug release (CIDR) protocol. On day 9 after CIDR removal, heifers (n = 7) with a corpus luteum (> 2 cm in size) received an embryo in the ipsilateral uterine horn. Pregnancy/ET was 57.1% (4/7). Economic advantage (increase in number of offspring) was realized comparing OPU-IVP and artificial insemination procedures. These ART technologies (OPU, IVP, and ET) can be utilized in genetically superior cows as value-added theriogenology service to increase their offspring.
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References
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2. Pieterse MC, Kappen KA, Kruip TA, et al: Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology. 1988;30:751-762. doi: 10.1016/0093-691x(88)90310-x
3. Ongaratto FL, Cedeño AV, Rodriguez-Villamil P, et al: Effect of FSH treatment on cumulus oocyte complex recovery by ovum pick up and in vitro embryo production in beef donor cows. Anim Reprod Sci 2020;214:106274. doi: 10.1016/j.anireprosci.2020.106274
4. Bó GA, Mapletoft RJ: Historical perspectives and recent research on superovulation in cattle. Theriogenology 2014;81:38-48. doi: 10.1016/j.theriogenology.2013.09.020
5. Egashira J, Ihara Y, Khatun H, et al: Efficient in vitro embryo production using in vivo-matured oocytes from superstimulated Japanese Black cows. J Reprod Dev 2019;65:183-190. doi: 10.1262/jrd.2018-155
6. Ferré LB, Kjelland ME, Strøbech LB, et al: Review: recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods. Animal;14:991-1004. doi: 10.1017/S1751731119002775
7. Viana JHM, Figueiredo ACS, Siqueira LGB: Brazilian embryo industry in context: pitfalls, lessons, and expectations for the future. Anim Reprod 2017;14:476-481. doi: 10.21451/1984-3143-AR989
8. Viana JHM., Siqueira LGB, Palhao MP et al: Features and perspectives of the Brazilian in vitro embryo industry. Anim Reprod 2012;9:12-18.
9. Demetrio D, Demetrio C, Oliveira M, et al: From Oocyte to calf: practical aspects of in vitro embryo production. Clinical Theriogenology 2022;14:193-201. doi: 10.58292/ct.v14.9671
10. Baruselli PS, Batista EOS, Vieira LM, et al: Factors that interfere with oocyte quality for in vitro production of cattle embryos: effects of different developmental & reproductive stages. Anim Reprod 2016;13:264-272. doi: 10.21451/1984-3143-AR861
11. Bó GA, Rogan DR, Mapletoft RJ: Pursuit of a method for single administration of pFSH for superstimulation in cattle: what we have learned. Theriogenology 2018;112:26-33. doi: 10.1016/j.theriogenology.2017.09.034
12. International Embryo Transfer Society manual, 4th edition. Available from: https://www.iets.org/.Publications/IETS-Manual [cited 6 June 2023].
13. Gutierrez K, Kasimanickam R, Tibary A, et al: Effect of reproductive tract scoring on reproductive efficiency in beef heifers bred by timed insemination and natural service versus only natural service. Theriogenology 2014;81:918-924. doi: 10.1016/j.theriogenology.2014.01.008
14. Bellows RA, Short RE, Richardson GV: Effects of sire, age of dam and gestation feed level on dystocia and postpartum reproduction. J Anim Sci 1982;55:18-27. doi: 10.2527/jas1982.55118x
15. Van Donkersgoed J, Ribble CS, Booker CW, et al: The predictive value of pelvimetry in beef cattle. Can J Vet Res 1993;57:170-175.
16. Thompson KW: Pelvic area as a tool for selection of beef replacement heifers. Master’s Thesis, University of Tennessee, Knoxville, TN, USA 1996. Available from: https://trace.tennessee.edu/utk_gradthes/6808 [Cited 7 June 1983].
17. Kasimanickam R, Schroeder S, Assay M, et al: Influence of temperament score and handling facility on stress, reproductive hormone concentrations, and fixed time AI pregnancy rates in beef heifers. Reprod Domest Anim 2014;49:775-782. doi: 10.1111/rda.12368
18. Leitman NR, Busch DC, Bader JF, et al: Comparison of protocols to synchronize estrus and ovulation in estrous-cycling and prepubertal beef heifers. J Anim Sci 2008;86:1808-18. doi: 10.2527/jas.2008-0970
19. White FJ, Wettemann RP, Looper ML, et al: Seasonal effects on estrous behavior and time of ovulation in nonlactating beef cows. J Anim Sci 2002;80:3053-9. doi: 10.2527/2002.80123053x
20. Sasser RG, Branen J, Howard J, et al: BioPRYN®, a measure of pregnancy-specific protein B for detection of pregnancy in ruminant animals. Proc Am Assoc Bovine Pract 2009:42:38-47. doi: 10.21423/aabppro20094248
21. Crowe AD, Lonergan P, Butler ST: Invited review: use of assisted reproduction techniques to accelerate genetic gain and increase value of beef production in dairy herds. J Dairy Sci 2021;104:12189-121206. doi: 10.3168/jds.2021-20281
22. Patterson DJ, Thomas JM, Bishop JM, et al: Control of estrus and ovulation in heifers. Proceedings of Applied Reproductive Strategies in beef cattle Stillwater, OK. 2014, p. 75-109.
23. Reese S, Pirez MC, Steele H, et al: The reproductive success of bovine sperm after sex-sorting: a meta-analysis. Sci Rep. 2021;11:17366. doi: 10.1038/s41598-021-96834-2
24. Bó GA, Cedeño A, Mapletoft RJ: Strategies to increment in vivo and in vitro embryo production and transfer in cattle. Anim Reprod 2019;16:411-422. doi: 10.21451/1984-3143-AR2019-0042
25. Morotti F, Sanches BV, Pontes JH, et al: Pregnancy rate and birth rate of calves from a large-scale IVF program using reverse-sorted semen in Bos indicus, Bos indicus-taurus, and Bos taurus cattle. Theriogenology 2014;81:696-701. doi: 10.1016/j.theriogenology.2013.12.002
26. Underwood SL, Bathgate R, Pereira DC, et al: Embryo production after in vitro fertilization with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm. Theriogenology 2010;73:97-102. doi:10.1016/j.theriogenology.2009.08.005
Published
2024-06-28
How to Cite
Boler R., & Kasimanickam R. (2024). Ovum pick up, in vitro embryo production, and embryo transfer as value-added theriogenology service in miniature Hereford cattle operation. Clinical Theriogenology, 16. https://doi.org/10.58292/CT.v16.10528
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Research Reports
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